Four hundred and seventy-five animals of the Canchim breed from EMBRAPA-UEPAE de Sao Carlos were analyzed by starch gel electrophoresis. 2. Albumin, amylase, transferrins, carbonic anhydrase, hemoglobin and nucleoside phosphorylase gene frequencies were compared with the same frequencies in the foundation breeds, Charolais and Indubrazil. 3. Theoretical values obtained considering the 5/8 European, 3/8 Zebu admixture are near the means calculated from our results and data from literature.