Dantas-Torres Filipe
Centro de Pesquisas Aggeu Magalhães, Fiocruz, Brazil.
Rev Inst Med Trop Sao Paulo. 2006 Jul-Aug;48(4):219-21. doi: 10.1590/s0036-46652006000400009.
The goal of this short communication is to report the uncommon presence of intracellular amastigotes of Leishmania in peritoneal fluid of a dog with leishmaniasis from Alagoas State, Brazil. Physical examination of an adult male rottweiler suspected to be suffering of leishmaniasis revealed severe loss of weight, ascitis, splenomegaly, moderately enlarged lymph nodes, onychogryphosis, generalized alopecia, skin ulcers on the posterior limbs, and conjunctivitis. Samples of bone marrow, popliteal lymph node, skin ulcer, and peritoneal fluid were collected and smears of each sample were prepared and stained with hematoxylin and eosin. Numerous amastigotes were detected in bone marrow, popliteal lymph node, and skin ulcer smears. Smears of peritoneal fluid revealed the unusual presence of several free and intracellular amastigotes of Leishmania. Future studies are needed to determine whether the cytology of ascitic fluid represents a useful tool for diagnosis Leishmania infection in ascitic dogs, particularly in those living in areas where canine leishmaniasis is enzootic.
本简短通讯的目的是报告在一只来自巴西阿拉戈斯州患利什曼病犬的腹腔液中罕见地出现利什曼原虫细胞内无鞭毛体。对一只疑似患利什曼病的成年雄性罗威纳犬进行体格检查发现体重严重减轻、腹水、脾肿大、淋巴结中度肿大、爪甲肥厚、全身性脱毛、后肢皮肤溃疡和结膜炎。采集了骨髓、腘淋巴结、皮肤溃疡和腹腔液样本，制备每个样本的涂片并用苏木精和伊红染色。在骨髓、腘淋巴结和皮肤溃疡涂片中检测到大量无鞭毛体。腹腔液涂片显示存在数只游离的和细胞内的利什曼原虫无鞭毛体，这种情况并不常见。需要进一步研究以确定腹水细胞学检查是否是诊断腹水型犬利什曼原虫感染的有用工具，特别是在犬利什曼病为地方流行的地区。
