Duarte Aulísia Maria Vieira, de Andrade Hélida Monteiro, do Monte Semíramis Jamil Hadad, de Toledo Vicente de Paulo Coelho Peixoto, Guimarães Tânia Mara Pinto Dabés
Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, University of Minas Gerais, Belo Horizonte, MG.
Rev Soc Bras Med Trop. 2006 Jul-Aug;39(4):385-7. doi: 10.1590/s0037-86822006000400014.
While testing 414 sera for the diagnosis of Chagas' disease, the conventional reactions of indirect hemagglutination, indirect immunofluorescence and the immunosorbent assay showed a sensitivity of 95.7%, 100% and 98.2% and a specificity of 98%, 98% and 96.4%, respectively, and an excellent association using Fisher's exact test. Chemiluminescence presented 100% sensitivity and 89.6% specificity, while PCR showed 100% specificity and 1.2% sensitivity. It is believed that the three conventional serological reactions are still adequate for diagnosing Chagas' disease.
在检测414份用于诊断恰加斯病的血清时，间接血凝、间接免疫荧光和免疫吸附试验的传统反应敏感性分别为95.7%、100%和98.2%，特异性分别为98%、98%和96.4%，采用Fisher精确检验显示相关性良好。化学发光法的敏感性为100%，特异性为89.6%，而聚合酶链反应（PCR）的特异性为100%，敏感性为1.2%。据信，这三种传统血清学反应对于诊断恰加斯病仍然足够。
