与用于诊断恰加斯病的传统血清学检测相关的化学发光和聚合酶链反应有效性评估。
Assessment of chemiluminescence and PCR effectiveness in relation to conventional serological tests for the diagnosis of Chagas' disease.
作者信息
Duarte Aulísia Maria Vieira, de Andrade Hélida Monteiro, do Monte Semíramis Jamil Hadad, de Toledo Vicente de Paulo Coelho Peixoto, Guimarães Tânia Mara Pinto Dabés
机构信息
Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, University of Minas Gerais, Belo Horizonte, MG.
出版信息
Rev Soc Bras Med Trop. 2006 Jul-Aug;39(4):385-7. doi: 10.1590/s0037-86822006000400014.
While testing 414 sera for the diagnosis of Chagas' disease, the conventional reactions of indirect hemagglutination, indirect immunofluorescence and the immunosorbent assay showed a sensitivity of 95.7%, 100% and 98.2% and a specificity of 98%, 98% and 96.4%, respectively, and an excellent association using Fisher's exact test. Chemiluminescence presented 100% sensitivity and 89.6% specificity, while PCR showed 100% specificity and 1.2% sensitivity. It is believed that the three conventional serological reactions are still adequate for diagnosing Chagas' disease.
在检测414份用于诊断恰加斯病的血清时，间接血凝、间接免疫荧光和免疫吸附试验的传统反应敏感性分别为95.7%、100%和98.2%，特异性分别为98%、98%和96.4%，采用Fisher精确检验显示相关性良好。化学发光法的敏感性为100%，特异性为89.6%，而聚合酶链反应（PCR）的特异性为100%，敏感性为1.2%。据信，这三种传统血清学反应对于诊断恰加斯病仍然足够。
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