Perfetti V, Borden P, Tao M H, Morrison S L, Kabat E A
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Mol Immunol. 1991 Apr-May;28(4-5):505-15. doi: 10.1016/0161-5890(91)90165-g.
We have characterized a monoclonal isogeneic antiidiotype, IdB5.7, from a BALB/c mouse immunized with the anti-alpha(1----6)dextran C57BL/6 45.21.1. It defined a hapten-inhibitable idiotope expressed on four of the 2 myeloma and 37 hybridoma anti-alpha(1----6)dextrans tested. Sequence comparison of Id+ and Id- anti-alpha(1----6)dextrans suggested that two extra amino acids at VH 100A and 100B and different residues at VH 101 abolish the expression of the idiotope in the Id- anti-alpha(1----6)dextrans. Sequence analysis of the VH of IdB5.7 showed a CDR1 longer than usual and a D segment in CDR3 formed by the fusion of two D minigenes. The IdB5.7 V kappa uses the V kappa 1 germline gene K5.1 with a few substitutions. The D-D fusion in VH CDR3 is a feature which has been reported in several other antiidiotypic antibodies.
我们从一只用抗α(1→6)葡聚糖C57BL/6 45.21.1免疫的BALB/c小鼠中鉴定出一种单克隆同基因抗独特型抗体IdB5.7。它定义了一种半抗原可抑制的独特型表位，在所测试的2种骨髓瘤和37种杂交瘤抗α(1→6)葡聚糖中，有4种表达该表位。Id+和Id-抗α(1→6)葡聚糖的序列比较表明，VH 100A和100B处的两个额外氨基酸以及VH 101处的不同残基消除了Id-抗α(1→6)葡聚糖中独特型表位的表达。IdB5.7的VH序列分析显示，其互补决定区1（CDR1）比通常的长，并且CDR3中的D片段由两个D小基因融合形成。IdB5.7的Vκ使用种系基因Vκ1 K5.1并带有一些替换。VH CDR3中的D-D融合是其他几种抗独特型抗体中已报道的一个特征。
