基于聚合酶链反应的福尔马林固定石蜡包埋脑切片中耐格里属感染的诊断
PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections.
作者信息
Schild Marc, Gianinazzi Christian, Gottstein Bruno, Müller Norbert
机构信息
Institute of Parasitology, University of Berne, Länggass-Strasse 122, CH-3012 Bern, Switzerland.
出版信息
J Clin Microbiol. 2007 Feb;45(2):564-7. doi: 10.1128/JCM.01357-06. Epub 2006 Nov 22.
Abstract
We developed a real-time PCR which allowed the highly sensitive detection of Naegleria fowleri in histological brain tissue sections from experimentally infected mice. This genus-specific small-subunit (18S) rRNA gene-based PCR can complement conventional (immuno-) histology for the diagnosis of primary amoebic meningoencephalitis in paraffin-embedded brain necropsy specimens that had been fixed in formalin buffered with phosphate-buffered saline.
摘要
我们开发了一种实时聚合酶链反应（PCR），可在实验感染小鼠的脑组织切片中高度灵敏地检测福氏耐格里阿米巴。这种基于属特异性小亚基（18S）核糖体RNA基因的PCR可补充传统（免疫）组织学方法，用于诊断在磷酸盐缓冲盐水缓冲的福尔马林中固定的石蜡包埋脑尸检标本中的原发性阿米巴脑膜脑炎。
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