Bransky Avishay, Korin Natanel, Nemirovski Yael, Dinnar Uri
Biomedical Department and Electrical Engineering Department, Technion, Haifa, Israel.
Microvasc Res. 2007 Jan;73(1):7-13. doi: 10.1016/j.mvr.2006.09.001. Epub 2006 Nov 22.
The deformability of erythrocytes is of great importance for oxygen delivery in the microcirculation [Lipowsky, H.H., 2005. Microvascular rheology and hemodynamics. Microcirculation 12, 5-15]. Aging of erythrocytes is associated with a reduction in deformability and also in size. The present work describes an automated cell analyzer which utilizes a glass microchannel and advanced image processing software. Erythrocytes suspended in a high viscosity medium are filmed flowing through the microchannel. Under these conditions, the cells assume different orientations and undergo varying deformations according to their location in the velocity profile. The cell analyzer enables the measurement of individual erythrocyte velocity, deformability and volume at varying depths within the microchannel. The volume of the cells is calculated based on the experimental data and a fluid mechanics model. The results obtained show that, on average, the deformability of the cells increases with increase in their size. Additionally, the behavior of RBCs in a microchannel is investigated, showing promising diagnostic possibilities.
红细胞的可变形性对于微循环中的氧气输送至关重要[利波夫斯基，H.H.，2005年。微血管流变学与血液动力学。微循环12，5 - 15]。红细胞衰老与可变形性降低以及尺寸减小有关。本研究描述了一种利用玻璃微通道和先进图像处理软件的自动细胞分析仪。悬浮在高粘度介质中的红细胞在流经微通道时被拍摄下来。在这些条件下，细胞根据其在速度分布中的位置呈现不同的取向并经历不同程度的变形。该细胞分析仪能够测量微通道内不同深度处单个红细胞的速度、可变形性和体积。细胞体积根据实验数据和流体力学模型计算得出。所得结果表明，平均而言，细胞的可变形性随其尺寸增大而增加。此外，还研究了红细胞在微通道中的行为，显示出有前景的诊断可能性。
