Hamnes Inger Sofie, Gjerde Bjørn Kåre, Forberg Torunn, Robertson Lucy Jane
Norwegian School of Veterinary Science, Department of Food Safety and Infection Biology, Section of Microbiology, Immunology and Parasitology, P.O. Box 8146 Dep, N-0033 Oslo, Norway.
Vet Parasitol. 2007 Mar 31;144(3-4):222-33. doi: 10.1016/j.vetpar.2006.10.011. Epub 2006 Nov 22.
Faecal samples from 684 litters of suckling piglets from 100 indoor swine herds from all regions of Norway were examined for the presence of Cryptosporidium and Giardia, using sucrose gradient flotation concentration and immunofluorescent staining. Thirty-one (31%) herds and 57 (8.3%) litters tested positive for Cryptosporidium, while 10 (1.5%) litters in 10 different herds (10%) tested positive for Giardia. Molecular characterisation of nine Cryptosporidium isolates demonstrated both C. suis and Cryptosporidium sp. pig genotype II. There was significantly more diarrhoea among the Cryptosporidium positive piglets than among the Cryptosporidium negative piglets. Diarrhoea was not observed amongst litters in which Cryptosporidium sp. pig genotype II was found. There was a significant difference in the Cryptosporidium prevalence between litters from different geographical areas of Norway. This study demonstrates that both Cryptosporidium (C. suis and Cryptosporidium sp. pig genotype II) and Giardia infections are prevalent among suckling piglets in Norway.
采用蔗糖梯度浮选浓缩和免疫荧光染色法，对来自挪威所有地区100个室内猪群的684窝哺乳仔猪的粪便样本进行了隐孢子虫和贾第虫检测。31个（31%）猪群和57窝（8.3%）仔猪隐孢子虫检测呈阳性，而10个不同猪群（10%）中的10窝（1.5%）仔猪贾第虫检测呈阳性。对9株隐孢子虫分离株进行分子特征分析，结果显示有猪隐孢子虫和猪隐孢子虫基因型II。隐孢子虫阳性仔猪的腹泻情况明显多于隐孢子虫阴性仔猪。在发现猪隐孢子虫基因型II的仔猪窝中未观察到腹泻。挪威不同地理区域仔猪窝的隐孢子虫感染率存在显著差异。本研究表明，隐孢子虫（猪隐孢子虫和猪隐孢子虫基因型II）和贾第虫感染在挪威哺乳仔猪中普遍存在。
