Kong Hyun Joon, Boontheekul Tanyarut, Mooney David J
Division of Engineering and Applied Science, Harvard University, Cambridge, MA 02138, USA.
Proc Natl Acad Sci U S A. 2006 Dec 5;103(49):18534-9. doi: 10.1073/pnas.0605960103. Epub 2006 Nov 21.
One of the fundamental interactions in cell biology is the binding of cell receptors to adhesion ligands, and many aspects of cell behavior are believed to be regulated by the number of these bonds that form. Unfortunately, a lack of methods to quantify bond formation, especially for cells in 3D cultures or tissues, has precluded direct probing of this assumption. We now demonstrate that a FRET technique can be used to quantify the number of bonds formed between cellular receptors and synthetic adhesion oligopeptides coupled to an artificial extracellular matrix. Similar quantitative relations were found between bond number and the proliferation and differentiation of MC3T3-E1 preosteoblasts and C2C12 myoblasts, although the relation was distinct for each cell type. This approach to understanding 3D cell-extracellular matrix interactions will allow one to both predict cell behavior and to use bond number as a fundamental design criteria for synthetic extracellular matrices.
细胞生物学中的基本相互作用之一是细胞受体与黏附配体的结合，并且细胞行为的许多方面被认为是由形成的这些键的数量所调节的。不幸的是，缺乏量化键形成的方法，特别是对于三维培养物或组织中的细胞，这妨碍了对这一假设的直接探究。我们现在证明，一种荧光共振能量转移（FRET）技术可用于量化细胞受体与偶联至人工细胞外基质的合成黏附寡肽之间形成的键的数量。在键的数量与MC3T3-E1前成骨细胞和C2C12成肌细胞的增殖及分化之间发现了类似的定量关系，尽管每种细胞类型的关系有所不同。这种理解三维细胞-细胞外基质相互作用的方法将使人们既能预测细胞行为，又能将键的数量用作合成细胞外基质的基本设计标准。
