一个调控衰老的NAC基因可提高小麦的籽粒蛋白质、锌和铁含量。
A NAC Gene regulating senescence improves grain protein, zinc, and iron content in wheat.
作者信息
Uauy Cristobal, Distelfeld Assaf, Fahima Tzion, Blechl Ann, Dubcovsky Jorge
机构信息
Department of Plant Sciences, University of California, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Science. 2006 Nov 24;314(5803):1298-301. doi: 10.1126/science.1133649.
Abstract
Enhancing the nutritional value of food crops is a means of improving human nutrition and health. We report here the positional cloning of Gpc-B1, a wheat quantitative trait locus associated with increased grain protein, zinc, and iron content. The ancestral wild wheat allele encodes a NAC transcription factor (NAM-B1) that accelerates senescence and increases nutrient remobilization from leaves to developing grains, whereas modern wheat varieties carry a nonfunctional NAM-B1 allele. Reduction in RNA levels of the multiple NAM homologs by RNA interference delayed senescence by more than 3 weeks and reduced wheat grain protein, zinc, and iron content by more than 30%.
摘要
提高粮食作物的营养价值是改善人类营养与健康的一种方式。我们在此报告Gpc-B1的定位克隆，Gpc-B1是一个与小麦籽粒蛋白质、锌和铁含量增加相关的数量性状基因座。野生小麦的原始等位基因编码一个NAC转录因子（NAM-B1），该因子会加速衰老并增加从叶片到发育中籽粒的养分再分配，而现代小麦品种携带一个无功能的NAM-B1等位基因。通过RNA干扰降低多个NAM同源基因的RNA水平，使衰老延迟超过3周，并使小麦籽粒蛋白质、锌和铁含量降低超过30%。
相似文献
1
A NAC Gene regulating senescence improves grain protein, zinc, and iron content in wheat.
Science. 2006 Nov 24;314(5803):1298-301. doi: 10.1126/science.1133649.
2
Functional characterization of GPC-1 genes in hexaploid wheat.
Planta. 2014 Feb;239(2):313-324. doi: 10.1007/s00425-013-1977-y. Epub 2013 Oct 30.
3
Haynaldia villosa NAM-V1 is linked with the powdery mildew resistance gene Pm21 and contributes to increasing grain protein content in wheat.
BMC Genet. 2016 Jun 14;17(1):82. doi: 10.1186/s12863-016-0391-4.
4
Wheat NAM genes regulate the majority of early monocarpic senescence transcriptional changes including nitrogen remobilization genes.
G3 (Bethesda). 2023 Feb 9;13(2). doi: 10.1093/g3journal/jkac275.
5
Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence.
BMC Plant Biol. 2014 Dec 19;14:368. doi: 10.1186/s12870-014-0368-2.
6
Swedish spring wheat varieties with the rare high grain protein allele of NAM-B1 differ in leaf senescence and grain mineral content.
PLoS One. 2013;8(3):e59704. doi: 10.1371/journal.pone.0059704. Epub 2013 Mar 21.
7
Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain.
J Exp Bot. 2009;60(15):4263-74. doi: 10.1093/jxb/erp257. Epub 2009 Oct 25.
8
Effect of the down-regulation of the high Grain Protein Content (GPC) genes on the wheat transcriptome during monocarpic senescence.
BMC Genomics. 2011 Oct 7;12:492. doi: 10.1186/1471-2164-12-492.
9
Divergent functions of orthologous NAC transcription factors in wheat and rice.
Plant Mol Biol. 2012 Mar;78(4-5):515-24. doi: 10.1007/s11103-012-9881-6. Epub 2012 Jan 26.
10
Overexpression of a NAC transcription factor delays leaf senescence and increases grain nitrogen concentration in wheat.
Plant Biol (Stuttg). 2015 Jul;17(4):904-13. doi: 10.1111/plb.12296. Epub 2015 Jan 16.
引用本文的文献
1
Identification of Salt Tolerance-Related Genes in Wheat Roots Based on RNA-Seq and Association Analysis.
Plants (Basel). 2025 Jul 27;14(15):2318. doi: 10.3390/plants14152318.
2
An introgression from Triticum timopheevii reduces grain protein content in winter wheat populations.
Plant Genome. 2025 Sep;18(3):e70090. doi: 10.1002/tpg2.70090.
3
Gene Family in : Genome-Wide Identification, Characterization, Expression Analysis, and Key Regulators Involved in Anthocyanin Biosynthesis.
Curr Issues Mol Biol. 2025 Jul 11;47(7):542. doi: 10.3390/cimb47070542.
4
Characterization of Semolina and Pasta Obtained from Hard Hexaploid Wheat ( L.) Developed Through Selection Assisted by Molecular Markers.
Foods. 2025 Jun 5;14(11):1990. doi: 10.3390/foods14111990.
5
Identification of Quantitative Trait Loci for Grain Quality Traits in a Pamyati Azieva × Paragon Bread Wheat Mapping Population Grown in Kazakhstan.
Plants (Basel). 2025 Jun 5;14(11):1728. doi: 10.3390/plants14111728.
6
Natural Variation of Regulates Plant Senescence in Tetraploid Potatoes ( L.).
Int J Mol Sci. 2025 May 5;26(9):4389. doi: 10.3390/ijms26094389.
7
Comparative Proteome and Weighted Gene Co-Expression Network Analyses Uncover the Mechanism of Wheat Grain Protein Accumulation in Response to Nitrogen Fertilizer Application.
Foods. 2025 Apr 24;14(9):1481. doi: 10.3390/foods14091481.
8
Progress and innovations of gene cloning in wheat and its close relatives.
Theor Appl Genet. 2025 Apr 29;138(5):106. doi: 10.1007/s00122-025-04897-w.
9
A novel, major, and validated QTL for grain zinc concentration independent of yield traits in tetraploid wheat.
Plant Genome. 2025 Jun;18(2):e70029. doi: 10.1002/tpg2.70029.
10
Micronutrient Biofortification in Wheat: QTLs, Candidate Genes and Molecular Mechanism.
Int J Mol Sci. 2025 Feb 28;26(5):2178. doi: 10.3390/ijms26052178.
本文引用的文献
1
The high grain protein content gene Gpc-B1 accelerates senescence and has pleiotropic effects on protein content in wheat.
J Exp Bot. 2006;57(11):2785-94. doi: 10.1093/jxb/erl047. Epub 2006 Jul 10.
2
AtNAP, a NAC family transcription factor, has an important role in leaf senescence.
Plant J. 2006 May;46(4):601-12. doi: 10.1111/j.1365-313X.2006.02723.x.
3
Physical map of the wheat high-grain protein content gene Gpc-B1 and development of a high-throughput molecular marker.
New Phytol. 2006;169(4):753-63. doi: 10.1111/j.1469-8137.2005.01627.x.
4
NAC transcription factors: structurally distinct, functionally diverse.
Trends Plant Sci. 2005 Feb;10(2):79-87. doi: 10.1016/j.tplants.2004.12.010.
5
Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana.
DNA Res. 2003 Dec 31;10(6):239-47. doi: 10.1093/dnares/10.6.239.
6
Microcolinearity between a 2-cM region encompassing the grain protein content locus Gpc-6B1 on wheat chromosome 6B and a 350-kb region on rice chromosome 2.
Funct Integr Genomics. 2004 Mar;4(1):59-66. doi: 10.1007/s10142-003-0097-3. Epub 2004 Jan 30.
7
Breeding for micronutrients in staple food crops from a human nutrition perspective.
J Exp Bot. 2004 Feb;55(396):353-64. doi: 10.1093/jxb/erh064.
8
Precise mapping of a locus affecting grain protein content in durum wheat.
Theor Appl Genet. 2003 Nov;107(7):1243-51. doi: 10.1007/s00122-003-1377-y. Epub 2003 Aug 16.
9
Microsatellite analysis reveals a progressive widening of the genetic basis in the elite durum wheat germplasm.
Theor Appl Genet. 2003 Sep;107(5):783-97. doi: 10.1007/s00122-003-1319-8. Epub 2003 Jul 4.
Zotero 插件