Smith F E, Rosen K M, Villa-Komaroff L, Weir G C, Bonner-Weir S
E. P. Joslin Research Laboratory, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215.
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6152-6. doi: 10.1073/pnas.88.14.6152.
Insulin-like growth factor I (IGF-I) mRNA expression was studied after 90% partial pancreatectomy in the rat to determine whether IGF-I was associated with pancreatic regeneration. The level of IGF-I mRNA was maximally increased (4-fold above control value) 3 days after pancreatectomy, but thereafter gradually decreased, returning to control levels by 14 days after surgery. By in situ hybridization, IGF-I mRNA in both pancreatectomized and sham-operated rats was localized to capillary endothelial cells, indicating that this is the site of IGF-I expression in the normal rat pancreas. However, enhanced IGF-I mRNA expression was localized to focal areas of regeneration unique to pancreatectomized rats. In these areas, epithelial cells of proliferating ductules and individual connective tissue cells expressed IGF-I, suggesting that IGF-I may play an important role in the growth or differentiation of pancreatic tissue.
研究了大鼠90%胰腺部分切除术后胰岛素样生长因子I（IGF-I）mRNA的表达情况，以确定IGF-I是否与胰腺再生有关。胰腺切除术后3天，IGF-I mRNA水平最高增加（比对照值高4倍），但此后逐渐下降，术后14天恢复到对照水平。通过原位杂交，胰腺切除大鼠和假手术大鼠的IGF-I mRNA均定位于毛细血管内皮细胞，表明这是正常大鼠胰腺中IGF-I的表达部位。然而，增强的IGF-I mRNA表达定位于胰腺切除大鼠特有的再生灶区域。在这些区域，增殖小导管的上皮细胞和单个结缔组织细胞表达IGF-I，提示IGF-I可能在胰腺组织的生长或分化中起重要作用。
