Hirasawa N, Watanabe M, Mue S, Tsurufuji S, Ohuchi K
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
Inflammation. 1991 Apr;15(2):117-26. doi: 10.1007/BF00917506.
The role of histamine in neutrophil infiltration and vascular permeability response in carrageenin air pouch inflammation in rats was examined. Injection of carrageenin solution into an air pouch induced a gradual increase in histamine content in the pouch fluid and histidine decarboxylase activity of pouch wall tissues, with a maximum attained at 24 h. Local administration of the H2 antagonists cimetidine and famotidine, but not the H1 antagonist pyrilamine, induced an increase in neutrophil infiltration at 24 h. Both types of histamine antagonists failed to suppress the vascular permeability response. In addition, H2 antagonists attenuated the inhibitory effect of indomethacin on neutrophil infiltration without affecting the indomethacin-induced suppression of vascular permeability response. These results suggest that histamine produced in the inflammatory locus exerts a downward regulation of neutrophil infiltration through H2 receptors but does not play any significant role in the vascular permeability response. Furthermore, the inhibition by indomethacin of neutrophil infiltration might be ascribed to the increase in histamine level in the pouch fluid.
研究了组胺在大鼠角叉菜胶气囊肿炎症中对中性粒细胞浸润和血管通透性反应的作用。向气囊肿内注射角叉菜胶溶液可导致囊液中组胺含量和囊壁组织中组氨酸脱羧酶活性逐渐增加，在24小时达到最大值。局部给予H2拮抗剂西咪替丁和法莫替丁，但不是H1拮抗剂吡拉明，可在24小时时诱导中性粒细胞浸润增加。两种类型的组胺拮抗剂均未能抑制血管通透性反应。此外，H2拮抗剂减弱了吲哚美辛对中性粒细胞浸润的抑制作用，而不影响吲哚美辛诱导的血管通透性反应抑制。这些结果表明，炎症部位产生的组胺通过H2受体对中性粒细胞浸润发挥下调作用，但在血管通透性反应中不发挥任何重要作用。此外，吲哚美辛对中性粒细胞浸润的抑制作用可能归因于囊液中组胺水平的增加。
