Moll R, Hage C, Thoenes W
Institute of Pathology, University of Mainz, Federal Republic of Germany.
Lab Invest. 1991 Jul;65(1):74-86.
The expression of intermediate filament proteins, particularly individual cytokeratins (CKs), vimentin, and glial filament protein, was immunohistochemically investigated using frozen sections and Carnoy-fixed, paraffin-embedded tissue from normal fetal and adult human kidneys as well as from pathologically altered kidneys. In fetal kidneys, the co-expression of CKs and vimentin was detected in the visceral and parietal epithelium of the glomerulus, the proximal tubules, the thin loops of Henle, and the collecting ducts. In contrast, in the tubules of normal adult kidneys, the presence of vimentin and CKs was nearly always mutually exclusive. While CKs 8 and 18 were present in all tubular epithelia, CKs 19 and 7 each exhibited a distinctive distribution pattern, there being a striking alteration between positive and negative segments and, not infrequently, intratubular heterogeneities. In certain segments, particular cell types (e.g., "plica cells," intercalated cells) could thus be recognized. In tubular epithelia altered by various injurious conditions, novel or enhanced expression of vimentin, CK 19 and CK 7, and, less frequently, CK 17 and glial filament protein was noted in certain segments. The increase in intermediate filament protein expression in altered (particularly proximal) tubules appeared to parallel the reduction in the degree of differentiation. Vimentin was never detected in distal tubules. The present results reveal a considerable similarity between the intermediate filament patterns in non-neoplastic proximal tubules of fetal and damaged kidney tissue and those in clear-cell and chromophilic renal cell carcinomas. They also serve to illustrate that the analysis of both fetal development and reactive cell changes may significantly contribute to our understanding of differentiation phenomena in malignant tumors.
采用免疫组织化学方法，利用冰冻切片以及取自正常胎儿和成人肾脏以及病变肾脏的经卡诺氏固定、石蜡包埋的组织，研究中间丝蛋白的表达情况，尤其是各种细胞角蛋白（CKs）、波形蛋白和神经胶质纤维酸性蛋白。在胎儿肾脏中，在肾小球的脏层和壁层上皮、近端小管、髓袢细段以及集合管中检测到CKs和波形蛋白的共表达。相比之下，在正常成人肾脏的小管中，波形蛋白和CKs的存在几乎总是相互排斥的。虽然CK8和CK18存在于所有肾小管上皮中，但CK19和CK7各自呈现出独特的分布模式，阳性和阴性节段之间存在显著变化，而且管内异质性并不少见。因此，在某些节段可以识别出特定的细胞类型（例如，“皱襞细胞”、闰细胞）。在因各种损伤情况而改变的肾小管上皮中，在某些节段发现波形蛋白、CK19和CK7以及较少见的CK17和神经胶质纤维酸性蛋白有新的表达或表达增强。改变的（尤其是近端）小管中中间丝蛋白表达的增加似乎与分化程度的降低平行。在远端小管中从未检测到波形蛋白。目前的结果揭示了胎儿和受损肾脏组织的非肿瘤性近端小管中的中间丝模式与透明细胞和嗜色性肾细胞癌中的中间丝模式之间存在相当大的相似性。它们还表明，对胎儿发育和反应性细胞变化的分析可能对我们理解恶性肿瘤中的分化现象有显著贡献。
