Holthöfer H, Miettinen A, Lehto V P, Lehtonen E, Virtanen I
Lab Invest. 1984 May;50(5):552-9.
Expression of cytokeratins and vimentin in developing and adult human kidney was studied by the indirect immunofluorescence technique using monoclonal and conventional antiintermediate filament antibodies. The undifferentiated cells of the metanephric mesenchyme expressed vimentin but not cytokeratins, whereas only cytokeratins could be visualized in the cells of induced renal vesicles. At the S-shaped body stage of nephrogenesis, the presumptive visceral and parietal cells of the developing glomeruli did not contain either detectable vimentin or cytokeratins, whereas cells of the tubular pole of the S-shaped body were stained within antibodies to cytokeratins. At a later stage the cuboidal visceral epithelial cells of the presumptive glomeruli were brightly positive for vimentin, but not for cytokeratins, and maintained their expression of vimentin during later stages of glomerulogenesis and in adult kidneys. Only some of the cells of the parietal epithelium, but not other glomerular elements, showed cytokeratin-specific staining. In fetal kidneys, the epithelial cells of proximal and distal tubules and of collecting ducts showed a cytoplasmic staining with two monoclonal antibodies to cytokeratins (PKK1 and PKK2), reacting with different cytokeratin polypeptides. In adult kidneys, a basolateral fluorescence in proximal and distal tubules was obtained with PKK1 antibodies, whereas PKK2 stained only the cells of the collecting ducts. Vimentin was not found in tubular epithelial cells at any developmental stage, whereas the cells of collecting ducts showed a transient expression of vimentin in fetal kidneys. Our results show a developmental stage-dependent pattern in the expression of the intermediate filament antigens in the cells of the kidney and show the exceptional expression of only vimentin in the visceral epithelial cells of human glomeruli.
利用单克隆抗体和传统抗中间丝抗体，通过间接免疫荧光技术研究了细胞角蛋白和波形蛋白在发育中和成人肾脏中的表达。后肾间充质的未分化细胞表达波形蛋白，但不表达细胞角蛋白，而在诱导的肾小泡细胞中只能观察到细胞角蛋白。在肾发生的S形期，发育中的肾小球的假定脏层和壁层细胞既未检测到波形蛋白也未检测到细胞角蛋白，而S形体肾小管极的细胞被细胞角蛋白抗体染色。在后期，假定肾小球的立方状脏层上皮细胞波形蛋白呈强阳性，但细胞角蛋白呈阴性，并在肾小球发生后期和成人肾脏中维持波形蛋白的表达。只有一些壁层上皮细胞，而不是其他肾小球成分，显示细胞角蛋白特异性染色。在胎儿肾脏中，近端和远端肾小管以及集合管的上皮细胞用两种针对细胞角蛋白的单克隆抗体（PKK1和PKK2）进行细胞质染色，这两种抗体与不同的细胞角蛋白多肽发生反应。在成人肾脏中，PKK1抗体在近端和远端肾小管中产生基底外侧荧光，而PKK2仅对集合管细胞染色。在任何发育阶段，肾小管上皮细胞中均未发现波形蛋白，而集合管细胞在胎儿肾脏中显示波形蛋白的短暂表达。我们的结果显示了肾脏细胞中中间丝抗原表达的发育阶段依赖性模式，并显示了人肾小球脏层上皮细胞中仅波形蛋白的特殊表达。
