Wang J P, Peng H C, Teng C M
Department of Medical Research, Taichung Veterans General Hospital, Republic of China.
Naunyn Schmiedebergs Arch Pharmacol. 1991 Apr;343(4):399-404. doi: 10.1007/BF00179045.
TMV F-IV, isolated from the venom of Trimeresurus mucrosquamatus (TMV), caused rat hind-paw edema in a dose-dependent manner. The maximum hind-paw swelling was reached at 1.5-2 h after subplantar injection of TMV F-IV. The edematous response caused by TMV F-IV was suppressed by the s.c. pretreatment with diphenhydramine, methysergide, acetylsalicylic acid or dexamethasone, and by the subplantar co-injection with FPL 55712, a SRS-A antagonist, and BN 52021 or L 652731, both PAF antagonists. Polymorphonuclear (PMN) leukocyte infiltration appeared within 1 h and gradually increased in the rat paw 3-6 h after edema induction. Compound 48/80 or methotrexate pretreatment also inhibited paw edema caused by TMV F-IV. In isolated mast cells, TMV F-IV increased the formation of PGE2 and LTB4 and caused a dose-dependent release of histamine and beta-glucuronidase. Since there are no significant differences in paw edema and mast cell degranulation responses between TMV F-IV and its DFP-modified analogue, the esterase activity may not be necessary in these models. These results indicate that mast cells. PMN leukocytes and some inflammatory mediators such as histamine, serotonin, arachidonate metabolites and PAF are involved in TMV F-IV induced paw edema.
从竹叶青蛇（TMV）毒液中分离出的TMV F-IV以剂量依赖的方式引起大鼠后爪水肿。足底注射TMV F-IV后1.5 - 2小时达到最大后爪肿胀。TMV F-IV引起的水肿反应可被苯海拉明、甲基麦角新碱、乙酰水杨酸或地塞米松的皮下预处理抑制，也可被与SRS-A拮抗剂FPL 55712以及PAF拮抗剂BN 52021或L 652731的足底联合注射抑制。多形核（PMN）白细胞浸润在水肿诱导后1小时内出现，并在大鼠爪中3 - 6小时逐渐增加。化合物48/80或甲氨蝶呤预处理也抑制了TMV F-IV引起的爪水肿。在分离的肥大细胞中，TMV F-IV增加了PGE2和LTB4的形成，并引起组胺和β-葡萄糖醛酸酶的剂量依赖性释放。由于TMV F-IV与其DFP修饰类似物之间在爪水肿和肥大细胞脱颗粒反应方面没有显著差异，在这些模型中酯酶活性可能不是必需的。这些结果表明肥大细胞、PMN白细胞以及一些炎症介质如组胺、5-羟色胺、花生四烯酸代谢产物和PAF参与了TMV F-IV诱导的爪水肿。
