Sato H, Suzuki A, Funahashi M, Takezawa T, Ogawa Y, Yoshizato K
Yoshizato MorphoMatrix Project, ERATO, JRDC, Hiroshima, Japan.
Wound Repair Regen. 1996 Jan-Mar;4(1):103-14. doi: 10.1046/j.1524-475X.1996.40117.x.
Phenotypic alterations of keloid-derived fibroblasts were characterized by comparison with the phenotypes of normal fibroblasts from the same patient. Explant cultures of keloids showed unique features. Keloid explants contracted considerably and reduced their size during culture, whereas the size of normal skin explants remained unchanged. Enlarged cells were found among fibroblasts which had grown out of all the explants and were morphologically distinct from fibroblasts; however, keloid explants produced many more of them than did the normal tissues. The growth rate of fibroblast colonies formed from normal explants was five times higher than keloid explants. Keloid fibroblasts which had been serially cultivated contracted lattices of collagen gels at a rate similar to normal fibroblasts. Proteins extracted from serially cultivated fibroblasts were mapped on polyacrylamide two-dimensional electrophoretic gels. No significant qualitative alterations in protein expression in keloid cells were found as compared with normal fibroblasts. However, some quantitative changes were found between the two. A computer-assisted image analyzer detected 151 polypeptide spots--50 spots (33%) of which increased their amounts in keloid cells, whereas 34 spots (22.5%) decreased in comparison with normal fibroblasts. Sixteen major polypeptides were identified as known proteins with the aid of time-of-flight mass spectrometry. The level of expression of these identified proteins was similar between normal and keloid cells, except stathmin whose expression was suppressed in keloid fibroblasts.
通过与同一患者正常成纤维细胞的表型进行比较，对瘢痕疙瘩来源的成纤维细胞的表型改变进行了表征。瘢痕疙瘩的外植体培养显示出独特的特征。瘢痕疙瘩外植体在培养过程中显著收缩并减小其大小，而正常皮肤外植体的大小保持不变。在从所有外植体生长出来的成纤维细胞中发现了增大的细胞，其形态与成纤维细胞不同；然而，瘢痕疙瘩外植体产生的此类细胞比正常组织多得多。由正常外植体形成的成纤维细胞集落的生长速率比瘢痕疙瘩外植体高五倍。连续传代培养的瘢痕疙瘩成纤维细胞收缩胶原凝胶晶格的速率与正常成纤维细胞相似。从连续传代培养的成纤维细胞中提取的蛋白质在聚丙烯酰胺二维电泳凝胶上进行图谱分析。与正常成纤维细胞相比，未发现瘢痕疙瘩细胞中蛋白质表达有明显的定性改变。然而，两者之间发现了一些定量变化。计算机辅助图像分析仪检测到151个多肽斑点，其中50个斑点（33%）在瘢痕疙瘩细胞中的含量增加，而与正常成纤维细胞相比，34个斑点（22.5%）减少。借助飞行时间质谱法鉴定出16种主要多肽为已知蛋白质。除了在瘢痕疙瘩成纤维细胞中表达受到抑制的微管相关蛋白2外，这些已鉴定蛋白质的表达水平在正常细胞和瘢痕疙瘩细胞之间相似。
