转录的结构基础：触发环在底物特异性和催化作用中的作用。
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis.
作者信息
Wang Dong, Bushnell David A, Westover Kenneth D, Kaplan Craig D, Kornberg Roger D
机构信息
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
出版信息
Cell. 2006 Dec 1;127(5):941-54. doi: 10.1016/j.cell.2006.11.023.
Abstract
New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.
摘要
RNA聚合酶II（pol II）转录复合物的新结构揭示了转录的一个可能关键。触发环在核苷酸添加位点的正确三磷酸核苷（NTP）下方摆动，封闭活性中心，并与NTP的碱基、糖、磷酸以及其他pol II残基形成广泛的相互作用网络。触发环中的一个组氨酸侧链通过这些相互作用精确地定位，可能实际上“触发”磷酸二酯键的形成。因此，识别和催化是耦合的，确保了转录的保真度。
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