Heus H A, Pardi A
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Science. 1991 Jul 12;253(5016):191-4. doi: 10.1126/science.1712983.
The most frequently occurring RNA hairpins in 16S and 23S ribosomal RNA contain a tetranucleotide loop that has a GNRA consensus sequence. The solution structures of the GCAA and GAAA hairpins have been determined by nuclear magnetic resonance spectroscopy. Both loops contain an unusual G-A base pair between the first and last residue in the loop, a hydrogen bond between a G base and a phosphate, extensive base stacking, and a hydrogen bond between a sugar 2'-end OH and a base. These interactions explain the high stability of these hairpins and the sequence requirements for the variant and invariant nucleotides in the GNRA tetranucleotide loop family.
16S和23S核糖体RNA中最常见的RNA发夹结构含有一个具有GNRA共有序列的四核苷酸环。GCAA和GAAA发夹结构的溶液结构已通过核磁共振光谱法确定。这两个环在环的第一个和最后一个残基之间都含有一个不寻常的G-A碱基对、一个G碱基与一个磷酸之间的氢键、广泛的碱基堆积以及一个糖2'-末端羟基与一个碱基之间的氢键。这些相互作用解释了这些发夹结构的高稳定性以及GNRA四核苷酸环家族中可变和不变核苷酸的序列要求。
