Oponowicz Agnieszka, Franczak Anita, Kurowicka Beata, Kotwica Genowefa
Department of Animal Physiology, Faculty of Biology, University of Warmia and Mazury in Olsztyn, Oczapowski 1A, 10-718 Olsztyn, Poland.
J Appl Genet. 2006;47(4):345-51. doi: 10.1007/BF03194644.
The aim of the present study was to investigate transcript localization of the oxytocin receptor (OTR) gene in different cells of the porcine uterus during luteolysis and early pregnancy (days 14-16) using in situ hybridization (ISH). OTR mRNA was localized in the uterine luminal epithelium (LEC), glandular epithelium (GEC), stromal cells (SC) of the endometrium, in the longitudinal muscle layer (LM) and circular muscle layer (CM) of the myometrium. The OTR transcript was quantified by optical density units of silver grains. The OTR transcript levels in the endometrium and myometrium were statistically higher during luteolysis than during early pregnancy (P<0.05). Besides, during luteolysis, the mRNA level was higher in the LEC, GEC of the endometrium and LM of the myometrium compared to that observed in the SC of endometrium and CM of the myometrium, respectively (P<0.05). In summary: 1) the level of OTR mRNA in uterine tissues is higher during luteolysis compared to early pregnancy, 2) the OTR transcript level in endometrial cells did not correspond to the sensitivity of these cells to oxytocin (OT), 3) the myometrial expression of the OTR gene is appropriate to control contractile activity and secretion of PG during luteolysis.
本研究的目的是使用原位杂交（ISH）技术，研究猪子宫在黄体溶解和妊娠早期（第14 - 16天）不同细胞中催产素受体（OTR）基因的转录本定位。OTR mRNA定位于子宫内膜的子宫腔上皮（LEC）、腺上皮（GEC）、基质细胞（SC），以及子宫肌层的纵行肌层（LM）和环行肌层（CM）。通过银颗粒的光密度单位对OTR转录本进行定量。黄体溶解期间子宫内膜和子宫肌层中的OTR转录本水平在统计学上高于妊娠早期（P<0.05）。此外，在黄体溶解期间，与子宫内膜SC和子宫肌层CM相比，子宫内膜LEC、GEC以及子宫肌层LM中的mRNA水平分别更高（P<0.05）。总之：1）与妊娠早期相比，黄体溶解期间子宫组织中OTR mRNA水平更高；2）子宫内膜细胞中OTR转录本水平与这些细胞对催产素（OT）的敏感性不相符；3）子宫肌层中OTR基因的表达适合在黄体溶解期间控制收缩活动和PG分泌。
