Kim S U, Rizzuto N
J Neuropathol Exp Neurol. 1975 Nov;34(6):531-41. doi: 10.1097/00005072-197511000-00007.
Well myelinated cultures of newborn mouse cerebellum, exposed to varying concentrations of sodium diethyldithiocarbamate (DDC), a heavy metal chelating agent, were examined by light and electron microscopy. DDC treatment of cultures for 24-48 hours produced swellings of axons and presynaptic endings, the morphological features characteristic of dystrophic axons. The axonal swellings contained an increased amount of endoplasmic reticulum, mitochondria and dense bodies. A continued exposure to DDC induced an extensive degeneration of axons and ensheathing myelin. Glial cells, on the other hand, were structurally intact even after a long-term exposure to DDC. It is suggested that DDC produces in myelinated CNS cultures an initial enlargement of axons and presynaptic endings and then continues to induce Wallerian degeneration in axons.
用光学显微镜和电子显微镜检查了新生小鼠小脑的充分髓鞘化培养物，这些培养物暴露于不同浓度的重金属螯合剂二乙基二硫代氨基甲酸钠（DDC）中。用DDC处理培养物24至48小时会导致轴突和突触前末梢肿胀，这是营养不良性轴突的形态学特征。轴突肿胀包含数量增加的内质网、线粒体和致密小体。持续暴露于DDC会导致轴突和髓鞘广泛退化。另一方面，即使长期暴露于DDC，神经胶质细胞的结构仍保持完整。有人提出，DDC在有髓鞘的中枢神经系统培养物中会使轴突和突触前末梢首先肿大，然后继续诱导轴突发生华勒氏变性。
