Tedeschi A, Arquati M, Lorini M, Miadonna A
Department of Internal Medicine, Infectious Diseases and Immunopathology, University of Milan, Ospedale Maggiore Policlinico, Italy.
Int Arch Allergy Appl Immunol. 1990;93(4):359-64. doi: 10.1159/000235266.
Effects of different extracellular Na+ and K+ concentrations (respectively, 135, 155, 220, 260 mM NaCl, and 2.7, 20, 50, 100 mM KCl) on IgE-dependent and IgE-independent histamine release from human basophils were examined. High extracellular Na+ and K+ concentrations were shown to reduce N-formyl-methionyl-leucyl-phenyl-alanine- (FMLP), but not anti-IgE- or Ca2+ ionophore A23187-induced histamine release. A high extracellular Ca2+ (7.2 mM CaCl2) concentration increased basophil response to anti-IgE and FMLP. The enhancement of FMLP- but not of anti-IgE-induced histamine release was antagonized by high extracellular Na+ and K+ concentrations. When leukocytes were suspended in isotonic choline chloride solutions (choline is a nonpermeant monovalent cation), an enhancement of anti-IgE- and FMLP-induced histamine release was observed. This suggests that monovalent cations, namely Na+ ions, at physiological concentrations, downregulate histamine release from human basophils. At high choline chloride concentrations, FMLP-, but not anti-IgE-induced histamine release was inhibited. Thus, the reduction of FMLP-evoked histamine secretion from human basophils seems to be due to hypertonicity and not to the type of monovalent cation, either permeant or nonpermeant, contained in extracellular milieu. The different effects of a hypertonic solution on anti-IgE and FMLP-induced histamine release are probably related to the different cell activation pathways triggered by the two stimuli.
研究了不同细胞外Na⁺和K⁺浓度（分别为135、155、220、260 mM NaCl和2.7、20、50、100 mM KCl）对人嗜碱性粒细胞IgE依赖性和IgE非依赖性组胺释放的影响。结果显示，高细胞外Na⁺和K⁺浓度可降低N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）诱导的组胺释放，但不影响抗IgE或Ca²⁺离子载体A23187诱导的组胺释放。高细胞外Ca²⁺（7.2 mM CaCl₂）浓度可增强嗜碱性粒细胞对抗IgE和FMLP的反应。高细胞外Na⁺和K⁺浓度可拮抗FMLP诱导的组胺释放增强，但不影响抗IgE诱导的组胺释放增强。当白细胞悬浮于等渗氯化胆碱溶液中（胆碱是一种非渗透性单价阳离子）时，可观察到抗IgE和FMLP诱导的组胺释放增强。这表明生理浓度的单价阳离子，即Na⁺离子，可下调人嗜碱性粒细胞的组胺释放。在高浓度氯化胆碱条件下，FMLP诱导的组胺释放受到抑制，但抗IgE诱导的组胺释放不受影响。因此，人嗜碱性粒细胞FMLP诱发的组胺分泌减少似乎是由于高渗性，而非细胞外环境中所含单价阳离子的类型（渗透性或非渗透性）。高渗溶液对抗IgE和FMLP诱导的组胺释放的不同影响可能与两种刺激触发的不同细胞激活途径有关。
