Yamato K, el-Hajjoui Z, Koeffler H P
Department of Medicine, UCLA School of Medicine, Cedars-Sinai Medical Centre 90024.
Leuk Res. 1991;15(7):551-8. doi: 10.1016/0145-2126(91)90022-l.
Experiments were undertaken to study expression of hematopoietic growth factor RNAs in mesenchymal cells from a variety of organs including bone marrow, foreskin, gingiva, and lung. Cells from each organ had negligible expression of RNAs coding for granulocyte (G), macrophage (M), and granulocyte-macrophage (GM) colony stimulating factor (CSF), interleukin 1 beta (IL-1 beta), and IL-6. Fibroblasts from each tissue had a comparable ability to express the same cytokine RNAs. Surprisingly, the stimuli for expression of G-CSF RNA was disparate from the stimuli for expression of the other cytokine RNAs. While IL-1 beta enhanced accumulation of G-CSF RNA, tumor necrosis factor alpha (TNF) and 12-O-tetradecanoylphorbol-13-acetate (TPA) did not. In contrast, IL-1 beta, TNF, and TPA equally stimulated increased levels of M-CSF, GM-CSF, IL-1 beta and IL-6 RNAs.
开展实验以研究造血生长因子RNA在来自多种器官（包括骨髓、包皮、牙龈和肺）的间充质细胞中的表达情况。来自每个器官的细胞中，编码粒细胞（G）、巨噬细胞（M）和粒细胞-巨噬细胞（GM）集落刺激因子（CSF）、白细胞介素1β（IL-1β）和IL-6的RNA表达量可忽略不计。来自每个组织的成纤维细胞表达相同细胞因子RNA的能力相当。令人惊讶的是，G-CSF RNA表达的刺激因素与其他细胞因子RNA表达的刺激因素不同。虽然IL-1β可增强G-CSF RNA的积累，但肿瘤坏死因子α（TNF）和12-O-十四烷酰佛波醇-13-乙酸酯（TPA）却不能。相反，IL-1β、TNF和TPA均能同等程度地刺激M-CSF、GM-CSF、IL-1β和IL-6 RNA水平升高。
