Uroz Stéphane, Oger Phil, Chhabra Siri Ram, Cámara Miguel, Williams Paul, Dessaux Yves
CNRS, ISV, Bâtiment 23, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Arch Microbiol. 2007 Mar;187(3):249-56. doi: 10.1007/s00203-006-0186-5. Epub 2006 Nov 29.
Comamonas strain D1 enzymatically inactivates quorum-sensing (QS) signal molecules of the N-acyl homoserine lactone (N-AHSL) family, and exhibits the broadest inactivation range of known bacteria. It degrades N-AHSL with acyl-side chains ranging from 4 to 16 carbons, with or without 3-oxo or 3-hydroxy substitutions. N-AHSL degradation yields HSL but not N-acyl homoserine: strain D1 therefore harbors an amidohydrolase activity. Strain D1 is the fifth bacterium species in which an N-AHSL amidohydrolase is described. Consistent with its N-AHSL degradation ability, strain D1 efficiently quenches various QS-dependent functions in other bacteria, such as violacein production by Chromobacterium violaceum and pathogenicity and antibiotic production in Pectobacterium.
丛毛单胞菌菌株D1能通过酶促作用使N-酰基高丝氨酸内酯（N-AHSL）家族的群体感应（QS）信号分子失活，并且在已知细菌中表现出最广泛的失活范围。它能降解酰基侧链含有4至16个碳的N-AHSL，这些侧链可有或没有3-氧代或3-羟基取代基。N-AHSL降解产生高丝氨酸内酯（HSL）而非N-酰基高丝氨酸：因此，菌株D1具有酰胺水解酶活性。菌株D1是第五种被描述具有N-AHSL酰胺水解酶的细菌物种。与其N-AHSL降解能力一致，菌株D1能有效淬灭其他细菌中各种依赖QS的功能，如紫色杆菌产生的紫菌素以及果胶杆菌的致病性和抗生素产生。
