School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China.
Int J Mol Sci. 2013 Aug 26;14(9):17477-500. doi: 10.3390/ijms140917477.
With the emergence of antibiotic-resistant strains of bacteria, the available options for treating bacterial infections have become very limited, and the search for a novel general antibacterial therapy has received much greater attention. Quorum quenching can be used to control disease in a quorum sensing system by triggering the pathogenic phenotype. The interference with the quorum sensing system by the quorum quenching enzyme is a potential strategy for replacing traditional antibiotics because the quorum quenching strategy does not aim to kill the pathogen or limit cell growth but to shut down the expression of the pathogenic gene. Quorum quenching enzymes have been identified in quorum sensing and non-quorum sensing microbes, including lactonase, acylase, oxidoreductase and paraoxonase. Lactonase is widely conserved in a range of bacterial species and has variable substrate spectra. The existence of quorum quenching enzymes in the quorum sensing microbes can attenuate their quorum sensing, leading to blocking unnecessary gene expression and pathogenic phenotypes. In this review, we discuss the physiological function of quorum quenching enzymes in bacterial infection and elucidate the enzymatic protection in quorum sensing systems for host diseases and their application in resistance against microbial diseases.
随着抗生素耐药菌株的出现，治疗细菌感染的可用选择变得非常有限，因此寻找新型的通用抗菌疗法受到了更多关注。群体感应淬灭可以通过触发致病性表型来控制群体感应系统中的疾病。通过群体感应淬灭酶对群体感应系统的干扰是替代传统抗生素的一种潜在策略，因为群体感应淬灭策略的目的不是杀死病原体或限制细胞生长，而是关闭致病基因的表达。已经在群体感应和非群体感应微生物中鉴定出群体感应淬灭酶，包括内酯酶、酰基酶、氧化还原酶和对氧磷酶。内酯酶在多种细菌物种中广泛保守，具有可变的底物谱。群体感应微生物中群体感应淬灭酶的存在可以减弱它们的群体感应，从而阻止不必要的基因表达和致病性表型。在这篇综述中，我们讨论了群体感应淬灭酶在细菌感染中的生理功能，并阐明了群体感应系统中酶的保护作用，以预防宿主疾病及其在抵抗微生物疾病中的应用。
