Ateka E M, Barg E, Njeru R W, Thompson G, Vetten H J
Institute of Plant Virology, Microbiology and Biosafety, Biologische Bundesanstalt für Land- und Forstwirtschaft (BBA), Braunschweig, Germany.
Arch Virol. 2007;152(3):479-88. doi: 10.1007/s00705-006-0879-8. Epub 2006 Nov 30.
Sweet potato virus 2 (SPV2) is a tentative member of the genus Potyvirus, family Potyviridae. In addition to the type isolate of SPV2 recently characterised in greater detail, twelve additional isolates of this virus were obtained from sweet potato clones originating from China, Portugal, South Africa and Zambia. Sequences of the coat protein (CP) gene and 3' non-translated region (NTR) were determined. Comparisons of the CP gene sequences of these isolates revealed nucleotide and amino acid sequence identities ranging from 81 to 99% and from 86 to 99%, respectively. Phylogenetic analysis of sequences distinguished several groups, which partially correlated with the geographic origin of the isolates, and indicated that some isolates from South Africa and a Zambian isolate are most distinct both in CP and 3'NTR sequences. Host range studies of a selected number of isolates revealed some differences in test plant reactions, which appeared to correlate to some extent with the geographic origin and molecular distinctness of the SPV2 isolates. The results strongly suggest the occurrence of biologically and genetically diverse strains of SPV2.
甘薯病毒2（SPV2）是马铃薯Y病毒科马铃薯Y病毒属的一个暂定成员。除了最近对SPV2的典型分离株进行了更详细的鉴定外，还从源自中国、葡萄牙、南非和赞比亚的甘薯克隆中获得了该病毒的另外12个分离株。测定了外壳蛋白（CP）基因和3'非翻译区（NTR）的序列。对这些分离株的CP基因序列进行比较，发现核苷酸和氨基酸序列同一性分别为81%至99%和86%至99%。序列的系统发育分析区分了几个组，这些组与分离株的地理来源部分相关，并表明一些来自南非的分离株和一个赞比亚分离株在CP和3'NTR序列上最为不同。对选定数量的分离株进行的寄主范围研究揭示了试验植物反应的一些差异，这些差异似乎在一定程度上与SPV2分离株的地理来源和分子差异相关。结果强烈表明存在生物学和遗传上不同的SPV2株系。
