中国甘薯褪绿矮化病毒（SPCSV）和 5 种侵染甘薯的马铃薯 Y 病毒科病毒的分子变异性。
Molecular variability of sweet potato chlorotic stunt virus (SPCSV) and five potyviruses infecting sweet potato in China.
机构信息
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
出版信息
Arch Virol. 2013 Feb;158(2):491-5. doi: 10.1007/s00705-012-1503-8. Epub 2012 Oct 10.
To obtain a better understanding of the molecular variation of sweet potato viruses in China, 131 samples were collected from symptomatic sweet potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of sweet potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect sweet potato in China, and three different strains of SPFMV and two of SPVG were found.
为了更好地了解中国甘薯病毒的分子变异情况，从有症状的甘薯植物中采集了 131 个样本，用于甘薯褪绿矮化病毒（SPCSV）的热休克蛋白 70（hsp70）基因序列和五种马铃薯 Y 病毒科病毒（SPFMV、SPVC、SPVG、SPLV 和 SPV2）的外壳蛋白（CP）基因序列的 RT-PCR 分析。获得的 hsp70 序列为存在两种不同的 SPCSV 株系提供了证据。对从样本中扩增的 CP 序列进行分析表明，在中国所有五种马铃薯 Y 病毒科病毒都侵染甘薯，发现了三种不同的 SPFMV 株系和两种 SPVG 株系。
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