Krieg M, Bartsch W, Thomsen M, Voigt K D
J Steroid Biochem. 1983 Jul;19(1A):155-61.
Growth and integrity of human prostatic epithelium is strongly dependent upon the adjacent stroma. Furthermore, the human prostate is under the control of sex hormones. Both facts prompted us to compare epithelium with stroma of human benign prostatic hyperplasia (BPH) and normal prostate (NPR) concerning their metabolism, binding and tissue concentrations of androgens and estrogens. In vitro metabolism was analyzed by t.l.c., androgen and estrogen binding sites were determined by a charcoal adsorption technique, androgen and estrogen tissue concentrations were measured by RIA. The main results are--(1) Metabolism: BPH stroma shows 2-3 times higher 5 alpha-reductase activity than epithelium. This high 5 alpha-reductase activity in BPH stroma dictates the differences between unseparated BPH and NPR, in the former the 5 alpha-reductase activity being 2 times higher. The 3 alpha(beta)-hydroxysteroid dehydrogenase (HSDH) activity is more evenly distributed between stroma and epithelium of BPH and NPR. The ratios of 5 alpha-reductase to HSDH activity in the various tissue fractions indicate that the highest enzymatically regulated 5 alpha-dihydrotestosterone (DHT) enrichment must occur in BPH stroma. (2) Tissue concentrations: unseparated BPH contains 2.2 times more DHT than NPR. About 70% of the total DHT content is found in nuclear fractions, whereby the nuclear DHT content of BPH stroma is significantly higher than that of BPH epithelium. In addition, nuclei of BPH stroma contain significantly more estradiol than epithelial nuclei. (3) Binding: the androgen receptor is evenly distributed between epithelium and stroma of BPH, while the estrogen receptor is preferably assayed in BPH stroma. These studies indicate that the BPH stroma is not only a preferential tissue for 5 alpha-reductase activity and DHT enrichment but also for nuclear estradiol accumulation.
人前列腺上皮的生长和完整性强烈依赖于相邻的基质。此外，人前列腺受性激素的控制。这两个事实促使我们比较人良性前列腺增生（BPH）和正常前列腺（NPR）的上皮与基质在雄激素和雌激素的代谢、结合及组织浓度方面的差异。通过薄层层析分析体外代谢，用活性炭吸附技术测定雄激素和雌激素结合位点，用放射免疫分析法测量雄激素和雌激素的组织浓度。主要结果如下：（1）代谢：BPH基质的5α-还原酶活性比上皮高2 - 3倍。BPH基质中这种高5α-还原酶活性决定了未分离的BPH与NPR之间的差异，前者的5α-还原酶活性高2倍。3α（β）-羟基类固醇脱氢酶（HSDH）活性在BPH和NPR的基质与上皮之间分布更为均匀。不同组织部分中5α-还原酶与HSDH活性的比值表明，最高的酶促调节5α-双氢睾酮（DHT）富集必定发生在BPH基质中。（2）组织浓度：未分离的BPH中的DHT含量比NPR多2.2倍。约70%的总DHT含量存在于细胞核部分，其中BPH基质的细胞核DHT含量显著高于BPH上皮。此外，BPH基质的细胞核中含有的雌二醇明显多于上皮细胞核。（3）结合：雄激素受体在BPH的上皮和基质中分布均匀，而雌激素受体在BPH基质中含量更高。这些研究表明，BPH基质不仅是5α-还原酶活性和DHT富集的优先组织，也是核雌二醇积累的优先组织。
