Shao Y
Institute of Virology, Chineses Academy of Preventive Medicine.
Zhonghua Liu Xing Bing Xue Za Zhi. 1991 Jun;12(3):129-35.
Blood were collected from HIV infected persons in epidemic area of HIV infection in Yunnan province for isolation of HIV. The coculture method was used for cultivating the virus and reverse transcriptase assay (RT) was the main method for detection of HIV. Of 25 seropositive, 24 asymptomatic and one PGL, 10 showed positive RT activity (greater than 5,000 cpm/ml and with a steadily increase, some to more than 40,000 cpm/ml). The results were confirmed by the detection of HIV1 p24 Ag (ELISA) and HIV1 POL and GAG gene sequence (PCR]. In accordance with the reports from other labs, the viruses isolated from these group of persons infect only PMCs, grew slowly with gradual increase of RT activity and caused no CPE. Efforts are making, at present, to rise the virus titer with better culture system. The amplified gene sequence of the isolates are under investigating.
从云南省艾滋病感染流行区的艾滋病感染者中采集血液用于分离艾滋病病毒。采用共培养法培养病毒，逆转录酶测定（RT）是检测艾滋病病毒的主要方法。在25例血清学阳性者中，24例无症状，1例患卡波西肉瘤，其中10例RT活性呈阳性（大于5000 cpm/ml且持续升高，有些超过40000 cpm/ml）。通过检测HIV1 p24抗原（ELISA法）和HIV1 POL及GAG基因序列（PCR法）证实了结果。根据其他实验室的报告，从这些人身上分离出的病毒仅感染原代单核细胞，生长缓慢，RT活性逐渐增加，且不引起细胞病变效应。目前正在努力通过更好的培养系统提高病毒滴度。正在对分离株的扩增基因序列进行研究。
