Xu Hui, Li Xuan, Yang Zheng-han, Xie Jing-xia
Department of Radiology, Peking University Third Hospital, 49 North Garden Road, Haidian District, Beijing, 100083, P. R. China.
Acad Radiol. 2006 Dec;13(12):1532-7. doi: 10.1016/j.acra.2006.09.001.
We used a 1.5-T MR scanner to investigate in vivo hydrogen 1 ((1)H) MRS to evaluate metabolic changes in the hepatocarcinogenesis experimental rat model.
Hepatocellular carcinoma (HCC) was induced by diethylnitrosamine in 70 treated rats with 20 normal rats used as controls. Single-voxel (1)H MRS is performed to obtained the relative choline-to-lipid (Cho/lipid) ratio. The liver and tumor tissues are incised for the histologic examination. Based on the histologic result, the progression of hepatocarcinogenesis of the animal model was divided into three stages: fibrosis stage, cirrhosis stage, and HCC stage. The mean (+/-SD) ratio values are calculated and compared at various stages between the treated group and the control group.
In control group, the calculated mean (+/-SD) Cho/lipid ratio was 0.15 +/- 0.05. With the progression of hepatocarcinogenesis, the Cho/lipid ratio increased significantly, to 0.18 +/- 0.05, 0.24 +/- 0.07, and 0.38 +/- 0.19, respectively.
The (1)H MRS is technically feasible for evaluation of the metabolic changes in the animal model. A significant increase in choline-containing compounds level was observed in the HCC stage in the treated group.
我们使用1.5-T磁共振成像扫描仪对氢-1（¹H）磁共振波谱进行体内研究，以评估肝癌发生实验大鼠模型中的代谢变化。
用二乙基亚硝胺诱导70只大鼠发生肝细胞癌（HCC），20只正常大鼠作为对照。采用单体素¹H磁共振波谱获取胆碱与脂质的相对比值（Cho/脂质）。切取肝脏和肿瘤组织进行组织学检查。根据组织学结果，将动物模型的肝癌发生进程分为三个阶段：纤维化阶段、肝硬化阶段和肝癌阶段。计算并比较治疗组和对照组在不同阶段的平均（±标准差）比值。
对照组计算得出的平均（±标准差）Cho/脂质比值为0.15±0.05。随着肝癌发生进程，Cho/脂质比值显著升高，分别达到0.18±0.05、0.24±0.07和0.38±0.19。
¹H磁共振波谱在评估动物模型代谢变化方面技术上可行。在治疗组的肝癌阶段观察到含胆碱化合物水平显著升高。
