Sakura H, Fujii T, Okamoto K
Department of Obstetrics and Gynecology, Hamamatsu Medical Center, Japan.
Exp Clin Endocrinol. 1991 Mar;97(1):91-6. doi: 10.1055/s-0029-1211044.
High concentration of human calcitonin (hCT) was found in an ovarian carcinoid by radioimmunoassay. The hCT value was not affected by the presence of protease inhibitors. To confirm the presence of hCT in an ovarian carcinoid, hCT was isolated by the Baghdiantz method. The molecular weight of the ovarian hCT was determined using Sephadex G-75 gel filtration. Though the molecular weight of the ovarian hCT was variable, 90% corresponded to that of the authentic hCT. The carcinoid cells were examined by immunoperoxidase techniques. Those composed of strumal and trabecular structure were all argyrophilic, but hCT was only found in strumal structure. Significant concentrations of hCT were also found in ovarian cancers.
通过放射免疫分析法在一例卵巢类癌中发现了高浓度的人降钙素（hCT）。hCT值不受蛋白酶抑制剂存在的影响。为了证实卵巢类癌中存在hCT，采用Baghdiantz方法分离出hCT。使用Sephadex G - 75凝胶过滤法测定卵巢hCT的分子量。尽管卵巢hCT的分子量存在差异，但90%与天然hCT的分子量相当。通过免疫过氧化物酶技术对类癌细胞进行检查。由滤泡状和小梁状结构组成的细胞均嗜银，但仅在滤泡状结构中发现了hCT。在卵巢癌中也发现了显著浓度的hCT。
