Zhao Jing, Jin Zhi-Qiang, Xu Bi-Yu
State Key Laboratory of Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Yi Chuan. 2006 Dec;28(12):1619-26. doi: 10.1360/yc-006-1619.
The development of improved Musa by conventional breeding remains to be a difficult endeavor because of the long generation times, various levels of ploidy, sterility of most edible cultivars and limited genetic variability. Therefore, genetic engineering may offer an alternative method for crop enhancement. The transformation system mediated by Agrobacterium tumefaciens has been used successfully to transform banana during the past 10 years. This article reviews recent progresses on transformation of banana mediated by Agrobacterium tumefaciens and summarizes the factors affecting transformation efficiency. Existing problems and its prospects are briefly discussed.
由于世代周期长、多倍体水平各异、大多数可食用品种不育以及遗传变异性有限，通过传统育种培育改良香蕉仍然是一项艰巨的任务。因此，基因工程可能为作物改良提供一种替代方法。在过去十年中，根癌农杆菌介导的转化系统已成功用于香蕉转化。本文综述了根癌农杆菌介导香蕉转化的最新进展，并总结了影响转化效率的因素。简要讨论了存在的问题及其前景。
