Zhang Su-Zhi, Rong Ting-Zhao
Key Laboratory of Crop Genetic Resources and Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an 625014, China E-mail:
Yi Chuan. 2008 Oct;30(10):1249-56. doi: 10.3724/sp.j.1005.2008.01249.
The Agrobacterium-mediated transformation system is more and more attractive to scientists of microbiology, molecular biology and crop genetics and breeding due to its unique advantages, which promote its development rapidly in the past decade. Great success has been achieved in the Agrobacterium-mediated genetic transformation system in maize (Zea mays L.), an important food-supply crop. This article highlights the advances of Agrobacterium-mediated transformation system of maize and the major factors of the transformation process. Then, comments on the problems remained and the prospects of this transformation system are made.
由于其独特的优势,农杆菌介导的转化系统对微生物学、分子生物学以及作物遗传育种领域的科学家越来越具有吸引力,这促使该系统在过去十年中迅速发展。在重要的粮食作物玉米(Zea mays L.)的农杆菌介导遗传转化系统方面已取得了巨大成功。本文着重介绍了玉米农杆菌介导转化系统的进展以及转化过程中的主要影响因素。随后,对该转化系统仍然存在的问题及前景进行了评述。