Wu Zhi-Hao, Wang Jian, He Fu-Chu
Beijing Institute of Radiation Medicine, Beijing 100850, China.
Yi Chuan. 2006 Dec;28(12):1627-32. doi: 10.1360/yc-006-1627.
The principle of yeast two-hybrid were summarized, the basis for large scale yeast two-hybrid screening of protein-protein interaction was given and the main application of yeast two-hybrid in large scale screening of protein-protein interaction was presented. The problems of large scale yeast two-hybrid screening of protein-protein interaction were also discussed. Because there were a lot of false positives and false negatives in the result of protein-protein interaction obtained by large-scale yeast two-hybrid screening, the employment of other methods to study protein-protein interaction in large scale in parallel was proposed if possible.
总结了酵母双杂交的原理，给出了大规模酵母双杂交筛选蛋白质-蛋白质相互作用的依据，并介绍了酵母双杂交在大规模筛选蛋白质-蛋白质相互作用中的主要应用。还讨论了大规模酵母双杂交筛选蛋白质-蛋白质相互作用存在的问题。由于大规模酵母双杂交筛选获得的蛋白质-蛋白质相互作用结果存在大量假阳性和假阴性，因此建议尽可能同时采用其他方法大规模研究蛋白质-蛋白质相互作用。
