Reuner K H, Schlegel K, Just I, Aktories K, Katz N
Institut für Klinische Chemie und Pathobiochemie, Universität Giessen, Germany.
FEBS Lett. 1991 Jul 29;286(1-2):100-4. doi: 10.1016/0014-5793(91)80950-8.
ADP-ribosylation of actin by Clostridium botulinum C2 toxin resulted in a depolymerization of filamentous F-actin and an increase of monomeric G-actin in cultured hepatocytes. Simultaneously the de novo synthesis of actin was largely reduced, while the synthesis of albumin and of other proteins was not significantly impaired. The specific decrease of actin mRNA to 30% of the control indicates a down-regulation of actin synthesis at a pretranslational level. On the other hand, treatment with the mycotoxin phalloidin resulted in an increase of F-actin and a decrease of monomeric G-actin. Under this condition the de novo synthesis of actin was specifically enhanced and the level of actin mRNA was increased to 600% of the control. The data suggest an autoregulatory control of the actin synthesis.
肉毒梭菌C2毒素对肌动蛋白的ADP核糖基化作用导致培养的肝细胞中丝状F-肌动蛋白解聚，单体G-肌动蛋白增加。同时，肌动蛋白的从头合成大幅减少，而白蛋白和其他蛋白质的合成未受到明显损害。肌动蛋白mRNA特异性降低至对照的30%，表明在翻译前水平上肌动蛋白合成受到下调。另一方面，用霉菌毒素鬼笔环肽处理导致F-肌动蛋白增加，单体G-肌动蛋白减少。在这种情况下，肌动蛋白的从头合成特异性增强，肌动蛋白mRNA水平增加至对照的600%。这些数据表明肌动蛋白合成存在自动调节控制。
