Serpinskaya A S, Denisenko O N, Gelfand V I, Bershadsky A D
Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
FEBS Lett. 1990 Dec 17;277(1-2):11-4. doi: 10.1016/0014-5793(90)80797-m.
We used the technique of scrape loading to introduce phalloidin into mouse embryo fibroblasts in mass culture. Phalloidin almost completely destroyed actin microfilament bundles, but the amount of polymerized cytoskeleton-associated actin was increased approximately two-fold and the amount of monomeric (Triton X-100 extractable) actin was significantly reduced. The major result of the present study is that the rate of actin synthesis in the phalloidin-treated cells was 2-3 times higher than in the control cells. Northern blot and translation in a cell-free system from rabbit reticulocytes showed that the actin mRNA level significantly increased as a result of phalloidin treatment.
我们运用刮取加载技术将鬼笔环肽大量引入处于群体培养状态的小鼠胚胎成纤维细胞中。鬼笔环肽几乎完全破坏了肌动蛋白微丝束，但聚合的细胞骨架相关肌动蛋白的量增加了约两倍，而单体（可被 Triton X - 100 提取的）肌动蛋白的量显著减少。本研究的主要结果是，经鬼笔环肽处理的细胞中肌动蛋白的合成速率比对照细胞高 2 - 3 倍。Northern 印迹分析以及来自兔网织红细胞的无细胞系统中的翻译实验表明，鬼笔环肽处理导致肌动蛋白 mRNA 水平显著升高。
