文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Ugarte M A, Rodríguez P

Laboratorio de Trombosis Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.

Int J Biochem. 1991;23(7-8):719-26. doi: 10.1016/0020-711x(91)90043-m.

Glycosyltransferase activity incorporating 14C-radioactivity from [14C]sucrose into endogenous acceptor was demonstrated in human dental plaque. 2. The enzyme was localized in dental plaque into two forms: (a) associated form to bacteria (pellet 10,000 g) and (b) released as an extracellular form (supernatant 10,000 g). 3. The reaction product was insoluble in 95% ethanol, soluble in trichloroacetic acid, and it was a mixture of saccharides with different sizes, as was demonstrated by column chromatography. 4. Exogenous activity with Dextran T-10 as substrate was also demonstrated, and it represented 9% of the total endogenous activity. 5. Characterization of the extracellular glycosyltransferase, and comparative results with glycosyltransferase secreted by oral bacteria in cultures medium are discussed.

在人类牙菌斑中证实了将[14C]蔗糖中的14C放射性掺入内源性受体的糖基转移酶活性。2. 该酶在牙菌斑中以两种形式存在：(a) 与细菌相关的形式（10,000 g离心沉淀）和(b) 以细胞外形式释放（10,000 g离心上清液）。3. 反应产物不溶于95%乙醇，可溶于三氯乙酸，通过柱色谱法证明它是不同大小糖类的混合物。4. 还证实了以葡聚糖T-10为底物的外源性活性，它占总内源性活性的9%。5. 讨论了细胞外糖基转移酶的特性以及与口腔细菌在培养基中分泌的糖基转移酶的比较结果。

Ugarte M A, Rodríguez P

Laboratorio de Trombosis Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.

Int J Biochem. 1991;23(7-8):719-26. doi: 10.1016/0020-711x(91)90043-m.

Glycosyltransferase activity incorporating 14C-radioactivity from [14C]sucrose into endogenous acceptor was demonstrated in human dental plaque. 2. The enzyme was localized in dental plaque into two forms: (a) associated form to bacteria (pellet 10,000 g) and (b) released as an extracellular form (supernatant 10,000 g). 3. The reaction product was insoluble in 95% ethanol, soluble in trichloroacetic acid, and it was a mixture of saccharides with different sizes, as was demonstrated by column chromatography. 4. Exogenous activity with Dextran T-10 as substrate was also demonstrated, and it represented 9% of the total endogenous activity. 5. Characterization of the extracellular glycosyltransferase, and comparative results with glycosyltransferase secreted by oral bacteria in cultures medium are discussed.

在人类牙菌斑中证实了将[14C]蔗糖中的14C放射性掺入内源性受体的糖基转移酶活性。2. 该酶在牙菌斑中以两种形式存在：(a) 与细菌相关的形式（10,000 g离心沉淀）和(b) 以细胞外形式释放（10,000 g离心上清液）。3. 反应产物不溶于95%乙醇，可溶于三氯乙酸，通过柱色谱法证明它是不同大小糖类的混合物。4. 还证实了以葡聚糖T-10为底物的外源性活性，它占总内源性活性的9%。5. 讨论了细胞外糖基转移酶的特性以及与口腔细菌在培养基中分泌的糖基转移酶的比较结果。