Pinheiro C E, Poletto M I, Pinheiro C F
Rev Odontol Univ Sao Paulo. 1989 Apr-Jun;3(2):368-70.
A simplified method for the partial purification and enzyme assay of glucosyltransferase from human dental plaque is described. The enzyme obtained has a reasonable specific activity for the assay of soluble and insoluble polysaccharides synthesis. This method may be useful for nonacquainted people with enzymological methods and of great utility in the in vitro evaluation of antiplaque substances.
本文描述了一种从人牙菌斑中部分纯化葡萄糖基转移酶并进行酶活性测定的简化方法。所获得的酶在测定可溶性和不溶性多糖合成方面具有合理的比活性。该方法对于不熟悉酶学方法的人可能有用，并且在抗牙菌斑物质的体外评估中具有很大的实用性。
