神经元去甲肾上腺素转运体在非洲爪蟾卵母细胞中的表达。

Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes.

作者信息

Coppeneur D, Lingen B, Sanders G, Dabauvalle M C, Bönisch H

机构信息

Department of Pharmacology and Toxicology University Bonn, Germany.

出版信息

Naunyn Schmiedebergs Arch Pharmacol. 1991 Mar;343(3):334-6. doi: 10.1007/BF00251136.

DOI:10.1007/BF00251136

PMID:1714043

Abstract

RNA isolated from both rat phaeochromocytoma (PC12) cells and bovine adrenal medulla was size-selected and injected into oocytes of Xenopus laevis. Expression of the neuronal noradrenaline transporter was assayed 3 days after injection by measuring the nisoxetine-sensitive noradrenaline uptake. A 2 kilobase fraction of either total RNA derived from both tissues or of polyadenylated mRNA obtained from bovine adrenal medulla caused Na(+)-dependent transport of 3H-noradrenaline in the injected oocytes.

摘要

从大鼠嗜铬细胞瘤（PC12）细胞和牛肾上腺髓质中分离出的RNA经大小筛选后注射到非洲爪蟾的卵母细胞中。注射3天后，通过测量对尼索西汀敏感的去甲肾上腺素摄取来检测神经元去甲肾上腺素转运体的表达。来自这两种组织的总RNA或从牛肾上腺髓质获得的聚腺苷酸化mRNA的2千碱基片段在注射的卵母细胞中引起了3H-去甲肾上腺素的钠依赖性转运。