神经元去甲肾上腺素转运体的分子层面

Molecular aspects of the neuronal noradrenaline transporter.

作者信息

Bönisch H, Paulus G, Martiny-Baron G, Lingen B, Coppeneur D

机构信息

Department of Pharmacology and Toxicology, University of Bonn, Federal Republic of Germany.

出版信息

J Neural Transm Suppl. 1991;34:11-7. doi: 10.1007/978-3-7091-9175-0_2.

DOI:10.1007/978-3-7091-9175-0_2

PMID:1726219

Abstract

The neuronal noradrenaline transporter was partially purified by means of low and high pressure liquid chromatography using anion exchange, gel filtration and lectin affinity columns. A protein characterized by a molecular weight of 50-53 kilodalton was enriched; it may represent the transporter or a component of it. In addition, a RNA fraction characterized by a mean size of 2 kilobases was isolated from PC12 rat phaeochromocytoma cells and from bovine adrenal medulla; this RNA fraction caused expression of the noradrenaline transporter after microinjection into Xenopus laevis oocytes.

摘要

通过使用阴离子交换、凝胶过滤和凝集素亲和柱的低压和高压液相色谱法，对神经元去甲肾上腺素转运体进行了部分纯化。富集了一种分子量为50 - 53千道尔顿的蛋白质；它可能代表转运体或其一个组分。此外，从大鼠嗜铬细胞瘤PC12细胞和牛肾上腺髓质中分离出了一个平均大小为2千碱基的RNA组分；将该RNA组分显微注射到非洲爪蟾卵母细胞后，可导致去甲肾上腺素转运体的表达。