Hawley Robert G, Ramezani Ali, Hawley Teresa S
Department of Anatomy and Cell Biology, The George Washington University Medical Center, Washington, DC, USA.
Methods Enzymol. 2006;419:149-79. doi: 10.1016/S0076-6879(06)19007-2.
Hematopoietic stem cells (HSCs) have the capacity to self-renew and the potential to differentiate into all of the mature blood cell types. The ability to prospectively identify and isolate HSCs has been the subject of extensive investigation since the first transplantation studies implying their existence almost 50 years ago. Despite significant advances in enrichment protocols, the continuous in vitro propagation of human HSCs has not yet been achieved. This chapter describes current procedures used to phenotypically and functionally characterize candidate human HSCs and initial efforts to derive permanent human HSC lines.
造血干细胞(HSCs)具有自我更新能力,并有可能分化为所有成熟血细胞类型。自近50年前首次进行暗示其存在的移植研究以来,前瞻性识别和分离造血干细胞的能力一直是广泛研究的主题。尽管在富集方案方面取得了重大进展,但人类造血干细胞的持续体外增殖尚未实现。本章描述了目前用于对候选人类造血干细胞进行表型和功能特征分析的程序,以及建立永久性人类造血干细胞系的初步努力。