Stoltzfus C M, Snyder P N
J Virol. 1975 Nov;16(5):1161-70. doi: 10.1128/JVI.16.5.1161-1170.1975.
Extracellular maturation of Bratislava 77(B77) sarcoma virus RNA involves a stabilization of linkage between 35S subunits since the Tm of 60 to 70S RNA in the presence of 0.5 M NaCl increases from 56 to 67.5 C as the age of the virus increases. This stabilization process is strongly temperature dependent; the rate at 45 C is increased fourfold over the rate at 37 C. As a result of the instability of the immature RNA, denaturation to subunits occurs at room temperature during phenol extraction. This dissociation can be prevented by increasing the NaCl concentration during the extraction. The data support a model which proposes that RNA subunits of oncornaviruses exist as assembled 60 to 70S RNA even in immature virions but that the linkages between subunits are stabilized as a function of time. These linkages appear to be maintained by nucleotide base pairing rather than by protein. However, isolated RNA does not undergo stabilization, suggesting that some other component of the virion is necessary for the process to occur.
布拉迪斯拉发77（B77）肉瘤病毒RNA的细胞外成熟涉及35S亚基之间连接的稳定，因为在0.5M NaCl存在下，随着病毒龄期增加，60至70S RNA的解链温度（Tm）从56℃升高至67.5℃。这种稳定过程强烈依赖于温度；45℃时的速率比37℃时增加四倍。由于未成熟RNA的不稳定性，在酚提取过程中，室温下会发生亚基变性。通过在提取过程中增加NaCl浓度可以防止这种解离。这些数据支持一个模型，该模型提出肿瘤病毒的RNA亚基即使在未成熟病毒粒子中也以组装的60至70S RNA形式存在，但亚基之间的连接会随着时间而稳定。这些连接似乎是通过核苷酸碱基配对而不是蛋白质来维持的。然而，分离的RNA不会发生稳定，这表明病毒粒子的某些其他成分对于该过程的发生是必需的。
