缺陷病毒粒子核心蛋白对禽肉瘤病毒RNA成熟的影响。
Influence of defective virion core proteins on RNA maturation with an avian sarcoma virus.
作者信息
Harms E, Rodhe W, Friis R R, Bauer H
出版信息
J Virol. 1977 Jan;21(1):419-22. doi: 10.1128/JVI.21.1.419-422.1977.
Abstract
The RNA of the avian sarcoma virus B77 temperature-sensitive mutant LA334 was investigated using electrophoretic analysis. The RNA from mutant virus grown at the nonpermissive temperature (42degrees C) showed a heterogeneous peak between 80 and 125S, and another at about 35S. The RNA of the mutant virus grown at the permissive temperature (35 degrees C) behaved like wild-type B77 virus RNA, exhibiting a major peak at 70S. The homology between the various RNA fractions and virus-specific DNA probe was determined, indicating that mutant virus grown at the nonpermissive temperature contains relatively large amounts of nonviral-specific RNA.
摘要
利用电泳分析研究了禽肉瘤病毒B77温度敏感突变体LA334的RNA。在非允许温度（42℃）下生长的突变病毒的RNA在80至125S之间显示出一个异质峰，另一个在约35S处。在允许温度（35℃）下生长的突变病毒的RNA表现得像野生型B77病毒RNA，在70S处有一个主峰。测定了各种RNA组分与病毒特异性DNA探针之间的同源性，表明在非允许温度下生长的突变病毒含有相对大量的非病毒特异性RNA。
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