B77禽肉瘤病毒基因组RNA亚基甲基化的证据。
Evidence of methylation of B77 avian sarcoma virus genome RNA subunits.
作者信息
Stoltzfus C M, Dimock K
出版信息
J Virol. 1976 May;18(2):586-95. doi: 10.1128/JVI.18.2.586-595.1976.
Abstract
B77 avian sarcoma virus RNA was labeled with (methyl-3H) methionine under conditions that prevent non-methyl incorporation of 3H radioactivity into purine rings. From the determined values for the extent of methylation of 4S RNA isolated from infected chicken embryo cells, it was estimated that 30 to 40S RNA subunits that results from heat denaturation of the 60 to 70S RNA contain approximately 21 methyl groups, of which 14 to 16 are present at internal positions as N6 -methyladenosine residues. In addition, each of the virion RNA subunits appears to contain about two methyl groups in the "capped" 5' -terminal structure m7G(5')ppp(5') gm. These properties are consistent with the hypothesis that the 30 to 40S genome RNA os oncornaviruses also serves an mRNA function in infected cells.
摘要
在防止3H放射性非甲基化掺入嘌呤环的条件下，用（甲基-3H）甲硫氨酸标记B77禽肉瘤病毒RNA。从感染鸡胚细胞中分离出的4S RNA的甲基化程度的测定值估计，由60至70S RNA热变性产生的30至40S RNA亚基含有约21个甲基，其中14至16个以N6-甲基腺苷残基的形式存在于内部位置。此外，每个病毒粒子RNA亚基在“加帽”的5'-末端结构m7G(5')ppp(5')gm中似乎含有约两个甲基。这些特性与以下假设一致，即肿瘤病毒的30至40S基因组RNA在感染细胞中也发挥mRNA的功能。
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