Minguela Alfredo, Miras Manuel, Bermejo Juan, Sánchez-Bueno Francisco, López-Alvarez María R, Moya-Quiles María R, Muro Manuel, Ontañón Jesús, Garía-Alonso Ana M, Parrilla Pascual, Alvarez-López Maria R
Immunology Services, Virgen de la Arrixaca University Hospital, Murcia, Spain.
Hum Immunol. 2006 Nov;67(11):884-93. doi: 10.1016/j.humimm.2006.06.005. Epub 2006 Jul 28.
Hepatitis B virus (HBV) and hepatitis C virus (HCV) generally reinfect liver graft early posttransplantation and lead to poorer graft and patient survivals. In the present study the influence of acute rejection (AR), HBV and HCV infections, and human leukocyte antigen (HLA) class-I compatibility on the expression of CD28 (in 237 liver recipients) and CD95 (in 114 liver recipients) on peripheral blood cells were evaluated by flow cytometry during the first month after transplantation. HBV/HCV infections induced strong CD95 upregulation on CD3+ lymphocytes. Maximal CD95 upmodulation was found in infected recipients showing partial HLA class-I compatibility. AR and virus reinfection could be distinguished because CD28 was upregulated on CD4+ lymphocytes only in recipients with AR, irrespective of their status regarding HBV/HCV infections. In conclusion, cytometric co-evaluation of CD95 and CD28 expression on peripheral blood lymphocytes could be useful to discriminate AR from cellular activation induced by viral reinfection of the liver graft.
乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）通常在肝移植术后早期再次感染肝脏移植物，并导致移植物和患者的生存率降低。在本研究中，通过流式细胞术评估了移植后第一个月内急性排斥反应（AR）、HBV和HCV感染以及人类白细胞抗原（HLA）I类相容性对237例肝移植受者外周血细胞上CD28表达以及114例肝移植受者外周血细胞上CD95表达的影响。HBV/HCV感染诱导CD3+淋巴细胞上CD95强烈上调。在显示部分HLA I类相容性的受感染受者中发现了最大程度的CD95上调。AR和病毒再感染可以区分，因为仅在发生AR的受者中，CD4+淋巴细胞上的CD28上调，而与他们的HBV/HCV感染状态无关。总之，对外周血淋巴细胞上CD95和CD28表达进行细胞计量学联合评估，可能有助于区分AR与肝脏移植物病毒再感染诱导的细胞活化。
