Wertz G W
J Virol. 1975 Nov;16(5):1340-4. doi: 10.1128/JVI.16.5.1340-1344.1975.
A method is described for radioactively labeling viral RNA and then quickly halting further incorporation of radioactive precursor into RNA so that the fate of the labeled RNA can be followed. Small complementary RNAs synthesized during vesicular stomatitis virus infection in the presence of cycloheximide do not metabolize to virion length molecules when protein synthesis inhibition is reversed.
本文描述了一种对病毒RNA进行放射性标记的方法,然后迅速阻止放射性前体进一步掺入RNA,以便追踪标记RNA的去向。在存在环己酰亚胺的情况下,水疱性口炎病毒感染期间合成的小互补RNA,当蛋白质合成抑制被逆转时,不会代谢为病毒体长度的分子。