White Jessica, Pacey-Miller Toni, Crawford Allison, Cordeiro Giovanni, Barbary Daniel, Bundock Peter, Henry Robert
Grain Foods CRC, Centre for Plant Conservation Genetics, Southern Cross University, PO Box 157, Lismore, NSW 2480, Australia.
Plant Biotechnol J. 2006 May;4(3):289-301. doi: 10.1111/j.1467-7652.2006.00181.x.
Serial analysis of gene expression (SAGE) was applied to the major cereal crop barley (Hordeum vulgare) to characterize the transcriptional profile of grain during the malting process. Seven SAGE libraries were generated from seed at different time points during malting, in addition to one library from dry mature seed. A total of 155,206 LongSAGE tags, representing 41,909 unique sequences, was generated. This study reports an in-depth analysis of the most abundant transcripts from each of eight specific time points in a malting barley time course. The 100 most abundant tags from each library were analysed to identify the putative functional role of highly abundant transcripts. The largest functional groups included transcripts coding for stress response and cell defence, ribosomal proteins and storage proteins. The most abundant tag represented B22EL8, a barley metallothionein, which showed significant up-regulation across the malting time course. Considerable changes in the abundance profiles of some of the highly abundant tags occurred at 24 h post-steeping, indicating that it may be an important time point for gene expression changes associated with barley seed germination.
基因表达序列分析（SAGE）被应用于主要谷类作物大麦（Hordeum vulgare），以表征麦芽制造过程中谷物的转录谱。除了来自干燥成熟种子的一个文库外，在麦芽制造过程中的不同时间点从种子中生成了七个SAGE文库。共生成了155,206个LongSAGE标签，代表41,909个独特序列。本研究报告了对麦芽大麦时间进程中八个特定时间点各自最丰富转录本的深入分析。分析了每个文库中最丰富的100个标签，以确定高度丰富转录本的推定功能作用。最大的功能组包括编码应激反应和细胞防御的转录本、核糖体蛋白和贮藏蛋白。最丰富的标签代表B22EL8，一种大麦金属硫蛋白，其在整个麦芽制造时间进程中显示出显著上调。一些高度丰富标签的丰度谱在浸泡后24小时发生了相当大的变化，表明这可能是与大麦种子萌发相关的基因表达变化的一个重要时间点。
