Stack S, Herickhoff L, Sherman J, Anderson L
Department of Biology, Colorado State University, Fort Collins 80523.
Biotech Histochem. 1991;1(2):69-78. doi: 10.3109/10520299109110553.
A technique is described for selectively silver staining nucleoli, active nucleolus organizers, nucleolar material attached to chromosomes, kinetochores, synaptonemal complexes, and chromosome cores in plant cells. The technique, called salt-nylon silver staining, involves spreading cells on glass slides, treating the cells with a solution of saline sodium citrate, and incubating the cells in a silver nitrate solution covered with nylon screen. Selected variables important for achieving reliable silver staining are considered.
本文描述了一种用于选择性银染植物细胞中的核仁、活跃的核仁组织区、附着于染色体的核仁物质、着丝粒、联会复合体和染色体核心的技术。该技术称为盐-尼龙银染法，包括将细胞铺展在载玻片上，用柠檬酸钠盐水溶液处理细胞，然后将细胞在覆盖有尼龙筛的硝酸银溶液中孵育。文中考虑了对实现可靠银染至关重要的选定变量。
