通过银染结合一种新的RNA特异性荧光染料观察到的核仁。
Nucleoli visualized by silver staining combined with a new RNA-specific fluorochrome.
作者信息
Wachtler F, Musil R
出版信息
Stain Technol. 1979 Sep;54(5):265-8. doi: 10.3109/10520297909110683.
PMID:93328
Abstract
A technique for distinguishing nucleolar components by light microscopy is described. Silver staining is used for localizing nuceolar-organizing regions. A new RNA-specific fluorochrome, 4''-6-bis(2'-imidazolinyl-4'-5'H)-2-phenyl-4'-phenoxyindole, visualizes the RNA-containing "partes fibrillares et granulares" of the nucleolus. Both staining procedures can be combined to analyze nucleolar structure and function by light microscopy.
摘要
本文描述了一种通过光学显微镜区分核仁成分的技术。银染用于定位核仁组织区。一种新的RNA特异性荧光染料，4''-6-双(2'-咪唑啉基-4'-5'H)-2-苯基-4'-苯氧基吲哚，可使核仁中含RNA的“纤维区和颗粒区”可视化。这两种染色方法可结合使用，通过光学显微镜分析核仁的结构和功能。
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