Migheli A, Attanasio A
2nd Neurologic Clinic, University of Turin, Italy.
Biotech Histochem. 1991;1(2):89-92. doi: 10.3109/10520299109110557.
Two simple methods are described for flat embedding of sections cut from paraffin blocks of brain tissue in the hydrophilic resin LR White. The ability of fresh resin to polymerize when added to already cured resin is exploited. In the first method, a tissue section with a drop of LR White is pressed to the bottom of a gelatin capsule using a blank block. In the second method, the section in a drop of resin is sandwiched between the sawed halves of a blank block. After curing, thin sections containing large areas of tissue can be collected and processed for immunocytochemistry.
本文描述了两种简单的方法，用于将从脑组织石蜡块切下的切片平嵌入亲水性树脂LR White中。利用了新鲜树脂加入已固化树脂时聚合的能力。第一种方法是，使用空白块将带有一滴LR White的组织切片压在明胶胶囊底部。第二种方法是，将一滴树脂中的切片夹在空白块锯开的两半之间。固化后，可收集含有大面积组织的薄片并进行免疫细胞化学处理。
