用于介观观察的未脱钙大鼠听泡的组织学制备。

Histological preparation of the undecalcified rat otic bulla for mesoscopic observations.

作者信息

Lataster L M, van Geest A, van den Berge H, Drukker J

机构信息

Department of Anatomy and Embryology, University of Limburg, Maastricht, The Netherlands.

出版信息

Biotech Histochem. 1991;1(2):93-8. doi: 10.3109/10520299109110558.

DOI:10.3109/10520299109110558

PMID:1714770

Abstract

Otic bullas of the rat, obtained by excision and formalin fixed, are successfully embedded in methylmethacrylate by dehydration and subsequent infiltration with plastic under vacuum. Sections 10 microns thick are obtained by cutting the trimmed and sandpapered acrylic blocks on an LKB multirange microtome. The sections are collected on adhesive tape and stained with a Trichrome stain (modified Weigert-van Gieson). Finally, the sections attached to the tape are mounted on microscope slides with glycerin-gelatin and sealed in the same medium. Serial sections are used for three-dimensional graphic reconstruction.

摘要

通过切除获得并经福尔马林固定的大鼠听泡，经脱水及随后在真空下用塑料浸润，成功地包埋于甲基丙烯酸甲酯中。在LKB多量程切片机上切割修整并经砂纸打磨的丙烯酸块，获得10微米厚的切片。切片收集在胶带上，用三色染色法（改良的魏格特-范吉森染色法）染色。最后，将附着在胶带上的切片用甘油明胶 mounting 在显微镜载玻片上，并用相同的介质密封。连续切片用于三维图形重建。