Adams D H, Hubscher S G, Shaw J, Johnson G D, Babbs C, Rothlein R, Neuberger J M
Liver Unit, Queen Elizabeth Hospital, Birmingham, United Kingdom.
Hepatology. 1991 Sep;14(3):426-31.
It has been suggested that immunological mechanisms involving lymphocyte-mediated damage are important in the characteristic bile-duct damage that occurs in primary biliary cirrhosis and primary sclerosing cholangitis. Because adhesion is necessary for the interaction of lymphocytes with their target structures, we have studied the expression of intercellular adhesion molecule 1, a ligand for the leukocyte adhesion receptor lymphocyte function-associated antigen 1 in the liver of patients with primary biliary cirrhosis and primary sclerosing cholangitis. Strong expression of intercellular adhesion molecule 1 was seen on interlobular bile ducts and proliferating bile ductules in both conditions. In primary biliary cirrhosis, medium-sized ducts, which are spared by the disease, were negative. Minimal bile-duct staining was seen in conditions in which bile-duct damage is not a major feature, such as nonbiliary cirrhosis and acute liver diseases. In patients with cirrhosis from any cause, strong expression of intercellular adhesion molecule 1 was detected on the periseptal hepatocytes adjacent to new connective tissue. The intensity of immunohistochemical staining was recorded using a semiquantitative visual scoring system that was subsequently validated quantitatively by confocal laser scanning microscopy. The expression/induction of intercellular adhesion molecule 1 on bile ducts may be important in the pathogenesis of bile-duct damage in primary biliary cirrhosis and primary sclerosing cholangitis and is further evidence to support an immune pathogenesis in these two conditions. Furthermore, the induction of intercellular adhesion molecule 1 on hepatocytes may be an important factor in the liver-cell damage and fibrosis that occur during the development of cirrhosis.
有人提出，涉及淋巴细胞介导损伤的免疫机制在原发性胆汁性肝硬化和原发性硬化性胆管炎中发生的特征性胆管损伤中起重要作用。由于黏附是淋巴细胞与其靶结构相互作用所必需的，我们研究了细胞间黏附分子1的表达，细胞间黏附分子1是白细胞黏附受体淋巴细胞功能相关抗原1在原发性胆汁性肝硬化和原发性硬化性胆管炎患者肝脏中的配体。在这两种情况下，小叶间胆管和增生的胆小管上均可见细胞间黏附分子1的强表达。在原发性胆汁性肝硬化中，未受该疾病影响的中等大小胆管呈阴性。在胆管损伤不是主要特征的情况下，如非胆汁性肝硬化和急性肝病，可见最小程度的胆管染色。在任何原因引起的肝硬化患者中，在与新结缔组织相邻的间隔周围肝细胞上检测到细胞间黏附分子1的强表达。使用半定量视觉评分系统记录免疫组织化学染色强度，随后通过共聚焦激光扫描显微镜进行定量验证。细胞间黏附分子1在胆管上的表达/诱导可能在原发性胆汁性肝硬化和原发性硬化性胆管炎胆管损伤的发病机制中起重要作用，并且是支持这两种情况下免疫发病机制的进一步证据。此外，肝细胞上细胞间黏附分子1的诱导可能是肝硬化发展过程中发生的肝细胞损伤和纤维化的重要因素。
